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. 2014 Jan 2;99(3):E427–E437. doi: 10.1210/jc.2013-3717

Figure 2.

Figure 2.

COUP-TFII is down-regulated by proinflammatory cytokines elevated in peritoneal fluid of individuals with endometriosis. A, Representative Western blot (upper panel) and quantitative results (lower panel, n = 4) show levels of COUP-TFII protein in normal endometrial stromal cells treated with peritoneal fluids (PFs) derived from women with [PF(Ec)] and without [PF(N)] endometriosis. *, Significant difference from control (Con). B–D, Representative Western blots (upper panel) and quantitative results (lower panel, n = 4) show levels of COUP-TFII in normal endometrial stromal cells treated with different doses of IL-1β (B), TNF-α (C), or TGF-β1 (D) for 24 hours. *, Significant difference from vehicle controls. E, Representative Western blot (upper panel) and quantitative result (lower panel, n = 4) show levels of COUP-TFII in normal endometrial stromal cells treated with IL-1β (IL; 0.01 ng/mL), TNF-α (TN; 0.1 ng/mL), TGF-β1 (TG; 0.01 ng/mL) or in combination (I/N/G) for 24 hours. *, Significant difference from vehicle control. F, Representative Western blots (upper panel) and quantitative results (low panel, n = 4) show levels of COUP-TFII in normal endometrial stromal cells treated with IL-1β in the presence or absence of recombinant IL-1β receptor antagonist (rIL1-ra; 200 ng/mL) for 24 hours. *, Significant difference from vehicle control (Con); #, significant difference from PBS-treated group. G, Representative Western blots (upper panel) and quantitative results (low panel, n = 4) show levels of COUP-TFII in normal endometrial stromal cells treated with TGF-β1 in the presence or absence of TGF-β receptor inhibitor (SB431542; 10 μM) for 24 hours. *, Significant difference from vehicle control (Con); #, significant difference from PBS-treated group.