Figure 2. Study of ARMC5 mutant-induced apoptosis in H295R cells.

The figure shows the immunofluorescence staining of FLAG and the cleaved caspase 3 in H295R cells 14 hours after transfection with the wild-type or mutant ARMC5 constructs. H295R cells were transfected with different ARMC5-FLAG constructs (wild-type). The cells were double-labeled with antibodies against the FLAG tag (red staining) and cleaved caspase 3 (green staining), in addition to nuclei staining by DAPI (blue). Double staining and altered morphology were observed with wild-type ARMC5, whereas transected cells with mutant ARMC5 did not express cleaved caspase 3 (white arrows).