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ChIP assays were performed in AtT20 cells treated with vehicle (Ros−) or R-roscovitine (Ros+) using PCR primer sets corresponding to the enhancer (ChIP1), the distal (ChIP2), and proximal (ChIP3) promoter region (A) with anti-E2F1 (E2F1) (B), antiacetyl histone H4 (AcH4) (C) antibodies, or negative control (IgG).
