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. 2017 Apr 17;12(4):e0175443. doi: 10.1371/journal.pone.0175443

Fig 3. Ad-ANXA1 transfected into PASMCs was strongly expressed and the protein was found in both the cytoplasm and nuclei.

Fig 3

(A) Morphology of PASMCs 24 h after transfection with Ad-ANXA1(magnification 100×). (B) Bright green fluorescence was observed in nearly all cells using an inverted fluorescence microscope following transfection with Ad-ANXA1 containing the green fluorescent protein (GFP) gene (magnification 100×). (C) The expression of the ANXA1 mRNA and protein in PASMCs 24 h after either the mock transfection or transfection with Ad-ANXA1 was determined by RT-PCR and western blotting analysis. GAPDH was used as an internal control in RT-PCR analysis and β-actin was used as an internal control in the Western blot analysis. Each data point represents the mean±S.E.M. of four independent experiments. *p<0.05 vs Group N.; Group N: Non-transfected group; Group T: transfected group; Group E: empty vector group.