Fig 2. Effects of DIO on the cell cycle distribution in A549/IR cells.

(A) A549/IR cells were treated with 10 μM DIO for 24 h, 6 Gy RT for 1 h, or a combination of DIO and RT, and then cell cycle distributions were analyzed with flow cytometry. The quantitative data of cell cycle distribution are shown. (B) Cell cycle-related proteins (CDK4/6, CyclinD1 and CDC2) were determined using western blot analysis. β-actin was used for the loading control.