Fig 6. Effect of tissue cryopreservation on induced pluripotent stem cell reprogramming of EDCs.
(A) Representative images of live iPSC colonies that express markers of pluripotent identity (SSEA-4 and Tra-1-60). Size bar is 100 μm. (B) Effect of tissue cryopreservation on pluripotent transcript expression of iPSC reprogrammed EDCs (mean ± SEM, n = 3 cell lines). (C) Effect of tissue cryopreservation on flow cytometry expression of pluripotent surface antigens (mean ± SEM, n = 3 cell lines).