Fig 4. Aldicarb potentiates wact-86 activity by inhibiting its GES-1-dependent hydrolysis.

(A) wact-86 dose-response assays, plus and minus 20 μM aldicarb, for wild-type worms and two strains harbouring ges-1 deletion alleles. (B) Quantification of wact-86 metabolite accumulation in wild-type worms incubated in wact-86 alone or in combination with aldicarb. (C) Quantification of wact-86 accumulation in wild-type worms incubated in wact-86 alone or in combination with aldicarb. For B and C, the area under the curve (AUC) values for the wact-86 parent and metabolite were calculated as in Fig 3. One and three asterisks indicate student’s t-test p < 0.05 and p < 0.001, respectively, compared to the aldicarb-untreated condition. Error bars represent the s.e.m.