Table 1. The HPLC solvent and flow rate gradients used herein.

Solvent A is 4.9:95:0.1 (ACN:H₂O:Acetic Acid); Solvent B is 95:4.9:0.1 (ACN: H₂O:Acetic Acid).

Time (min)	Solvent	Flow Rate (ml/min)
0.00	Solvent A: 85% Solvent B: 15%	1.5
0.15	Solvent A: 85% Solvent B: 15%	2.0
3.20	Solvent A: 30% Solvent B: 70%	2.0
4.25	Solvent B: 100%	3.0