Table 1.
COXIV zipcode interacting proteins identified by RNA affinity purification and mass spectrometry studies.
| Accession # | Gene Symbol | # of distinct peptides | % Coverage | Protein Score | Peptides |
|---|---|---|---|---|---|
| Q9UII2 | ATIF1_HUMAN | 4 | 49.06 | 131.18 | EAGGAFGKR; LQKEIERHK; GSDQSENVDRGAGSIR; EQAEEERYFR |
| P56385 | ATP5I_HUMAN | 4 | 55.1 | 160.29 | YSALFLGVAYGATR; IARELAEDDSILK;VPPVQVSPLIK; ELAEDDSILK |
| P48047 | ATPO_HUMAN | 12 | 48.8 | 246 | FSPLTTNLINLLAENGR; VAASVLNPYVK; SFLSQGQVLKLEAK; QNKLEQVEKELLR; SLNDITAKER; VAASVLNPYVKR; TDPSILGGMIVR; SFLSQGQVLK; VAQILKEPK; LVRPPVQVYGIEGR; FAKLVRPPVQVYGIEGR; ERFSPLTTNLINLLAENGR |
| Q00535 | CDK5_HUMAN | 3 | 10.27 | 72.68 | GLGFCHSR; LADFGLAR; LNATGRDLLQNLLK |
| Q14011 | CIRBP_HUMAN | 3 | 47.67 | 186.64 | SSGGSYRDSYDSYATHNE; SGGYGGSRDYYSSR; YGQISEVVVVKDRETQR |
| P62633 | CNBP_HUMAN | 8 | 53.11 | 226.71 | CGESGHLAKDCDLQEDACYNCGR; DCDLQEDACYNCGR; CGETGHVAINCSK; EQCCYNCGKPGHLAR; CGESGHLAR; SSNECFKCGR; TSEVNCYR; DCDHADEQKCYSCGEFGHIQKDCTK |
| P14854 | CX6B1_HUMAN | 3 | 61.63 | 99.77 | GGDISVCEWYQR; TAPFDSRFPNQNQTR; VYQSLCPTSWVTDWDEQRAEGTFPGKI |
| P99999 | CYC_HUMAN | 5 | 40.95 | 220.63 | KTGQAPGYSYTAANK; TGQAPGYSYTAANKNK; TGQAPGYSYTAANK; KTGQAPGYSYTAANKNK; ADLIAYLK |
| O43602 | DCX_HUMAN | 4 | 11.11 | 133.46 | SFDALLADLTR; SLSDNINLPQGVR; TAHSFEQVLTDITEAIKLETGVVKK; KLYTLDGK |
| Q86XP3 | DDX42_HUMAN | 2 | 4.9 | 70.75 | DILIDPIRVVQGDIGEANEDVTQIVEILHSGPSK; SHFVAASLSNQK |
| Q7L014 | DDX46_HUMAN | 2 | 2.33 | 85.1 | RILSKPIEVQVGGR; LLVATSVAAR |
| O43143 | DHX15_HUMAN | 5 | 13.58 | 203 | SNLGSVVLQLK; HQSFVLVGETGSGK; TLATDILMGVLKEVVR; IRVESLLVTAISK; RGVACTQPR |
| P25685 | DNJB1_HUMAN | 5 | 19.12 | 122.03 | KKQDPPVTHDLR; GASDEEIKR; SIRNEDKILTIEVK; DGSDVIYPAR; SIRNEDKILTIEVK |
| O14531 | DPYL4_HUMAN | 7 | 40.73 | 258.31 | VAVGSDADLVIWNPK; GAPAVVISQGR; NLHQSGFSLSGSQADDHIAR; KTFPDFVYKR; THNLNVEYNIFEGVECR; LLELGITGPEGHVLSHPEEVEAEAVYR; IVNDDQSFYADVHVEDGLIKQIGENLIVPGGIK |
| Q9BPU6 | DPYL5_HUMAN | 3 | 20.39 | 158.82 | THCPIYLVNVSSISAGDVIAAAK; VHAENGELVAEGAKEALDLGITGPEGIEISRPEELEAEATHR; CHGVPLVTISR |
| O60869 | EDF1_HUMAN | 6 | 50 | 171.8 | SKQAILAAQR; GLTQKDLATK; WAAGQNKQHSITK; GKDIGKPIEKGPR; GLTQKDLATKINEKPQVIADYESGR; AIPNNQVLGKIER |
| P06733 | ENOA_HUMAN | 5 | 24.42 | 244.32 | SGETEDTFIADLVVGLCTGQIK; AGAVEKGVPLYR; NFRNPLAK; AVEHINKTIAPALVSK; NVINGGSHAGNK |
| Q92945 | FUBP2_HUMAN | 8 | 13.22 | 208.42 | IGGGIDVPVPR; IINDLLQSLR; HSVGVVIGR; AGLVIGKGGETIKQLQER; IGGDAATTVNNSTPDFGFGGQKR; GGETIKQLQER; GSPQQIDHAK; SVSLTGAPESVQK |
| P35637 | FUS_HUMAN | 4 | 13.31 | 123.71 | AAIDWFDGKEFSGNPIKVSFATR; DQGSRHDSEQDNSDNNTIFVQGLGENVTIESVADYFK; AAIDWFDGKEFSGNPIK; QIGIIKTNKK |
| P09429 | HMGB1_HUMAN | 17 | 73.02 | 328.76 | IKGEHPGLSIGDVAK; KHPDASVNFSEFSK; YEKDIAAYR; GEHPGLSIGDVAK; RPPSAFFLFCSEYRPK; EKYEKDIAAYR; IKGEHPGLSIGDVAKK; HPDASVNFSEFSK; GKPDAAKKGVVK; KGVVKAEK; GKFEDMAKADK; GKGDPKKPR; KHPDASVNFSEFSKK; YEKDIAAYRAK; TYIPPKGETK; LGEMWNNTAADDKQPYEK; FKDPNAPKRPPSAFFLFCSEYRPK; GKFEDMAK; FKDPNAPK |
| P26583 | HMGB2_HUMAN | 17 | 71.77 | 318.98 | KKNEPEDEEEEEEEEDEDEEEEDED; IKSEHPGLSIGDTAK; SEHPGLSIGDTAK; YEKDIAAYR; EKYEKDIAAYR; IKSEHPGLSIGDTAKK; SEAGKKGPGRPTGSK; RPPSAFFLFCSEHRPK; SEHPGLSIGDTAKK; HPDSSVNFAEFSKK; YEKDIAAYRAK; DKQPYEQKAAK; SKFEDMAK; DKQPYEQK; KHPDSSVNFAEFSKK; NYVPPKGDKK |
| P31942 | HNRH3_HUMAN | 3 | 16.76 | 139.61 | STGEAFVQFASK; EIAENALGKHKER; HNGPNDASDGTVR |
| P61978 | HNRPK_HUMAN | 6 | 20.73 | 193.72 | ALRTDYNASVSVPDSSGPER; DLAGSIIGK; DLAGSIIGKGGQR; GSYGDLGGPIITTQVTIPK; GSYGDLGGPIITTQVTIPKDLAGSIIGK; HESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVK |
| P55145 | MANF_HUMAN | 2 | 14.84 | 103.32 | YAPKAASARTDL; LRPGDCEVCISYLGR |
| P46821 | MAP1B_HUMAN | 5 | 7.46 | 247.04 | SVGNTIDPVILFQK; TSDVGGYYYEKIER; SWDTNLIECNLDQELKLFVSR; AIGNIELGIR; TPDTSTYCYETAEKITR |
| P27816 | MAP4_HUMAN | 6 | 9.81 | 217.03 | TAGPIASAQKQPAGK; TTTLSGTAPAAGVVPSR; SVPADLSRPK; TTTAAAVASTGPSSR; LATNTSAPDLKNVR; ASPSKPASAPASR |
| P40926 | MDHM_HUMAN | 7 | 32.84 | 219.99 | AKVAVLGASGGIGQPLSLLLK; TIIPLISQCTPKVDFPQDQLTALTGR; AGAGSATLSMAYAGAR; AKVAVLGASGGIGQPLSLLLKNSPLVSR; LTLYDIAHTPGVAADLSHIETK; NSPLVSRLTLYDIAHTPGVAADLSHIETK |
| Q15233 | NONO_HUMAN | 3 | 11.68 | 160.31 | NLPQYVSNELLEEAFSVFGQVER; AVVIVDDRGR; AAPGAEFAPNKR |
| Q99497 | PARK7_HUMAN | 4 | 35.4 | 76.89 | GAEEMETVIPVDVMR; VTVAGLAGKDPVQCSR; DVVICPDASLEDAKK; EILKEQENR |
| P09874 | PARP1_HUMAN | 15 | 27.32 | 284.79 | VVSEDFLQDVSASTK; TTNFAGILSQGLR; SLQELFLAHILSPWGAEVKAEPVEVVAPR; TLGDFAAEYAK; HASHISKLPK; NTHATTHNAYDLEVIDIFKIER; VVDRDSEEAEIIRK; TAEAGGVTGKGQDGIGSKAEK; SLQELFLAHILSPWGAEVK; VCSTNDLKELLIFNK; SANYCHTSQGDPIGLILLGEVALGNMYELK; SKLPKPVQDLIK; GFSLLATEDKEALKK; AESSDKLYRVEYAK; NREELGFRPEYSASQLK |
| P62136 | PP1A_HUMAN | 3 | 18.79 | 165.98 | SDSEKLNLDSIIGR; AHQVVEDGYEFFAKR; FLHKHDLDLICR |
| Q9Y5S9 | RBM8A_HUMAN | 2 | 7.47 | 70.14 | GRGFGSEEGSR; GFGSEEGSRAR |
| P22626 | ROA2_HUMAN | 10 | 33.99 | 252.23 | YHTINGHNAEVR; RAVAREESGKPGAHVTVK; EESGKPGAHVTVK; YHTINGHNAEVRK; SGRGGNFGFGDSR; GGGGNFGPGPGSNFR; GGGGNFGPGPGSNFRGGSDGYGSGR; GFGFVTFDDHDPVDKIVLQK; AVAREESGKPGAHVTVK; ALSRQEMQEVQSSR |
| P51991 | ROA3_HUMAN | 2 | 18.52 | 176.61 | SSGSPYGGGYGSGGGSGGYGSR; GFAFVTFDDHDTVDKIVVQK |
| Q9NVA2 | SEP11_HUMAN | 2 | 11.35 | 144.46 | SYELQESNVR; AVAVGRPSNEELR |
| Q15019 | SEPT2_HUMAN | 8 | 40.72 | 266.01 | TVQIEASTVEIEER; SKQQPTQFINPETPGYVGFANLPNQVHR; ASIPFSVVGSNQLIEAK; TIISYIDEQFER; GRLYPWGVVEVENPEHNDFLK; AIHNKVNIVPVIAK; GRLYPWGVVEVENPEHNDFLKLR; STLINSLFLTDLYPERVIPGAAEKIER |
| Q14141 | SEPT6_HUMAN | 2 | 8.99 | 122.18 | AATDIARQVGEGCR; SLDDEVNAFKQR |
| Q16181 | SEPT7_HUMAN | 3 | 17.16 | 173 | ILEQQNSSR; STLINSLFLTDLYSPEYPGPSHR; LHEKVNIIPLIAK |
| Q9UHD8 | SEPT9_HUMAN | 9 | 26.28 | 260.61 | SDPAVNAQLDGIISDFEALKR; SITHDIEEKGVR; STLINTLFK; SVQPTSEERIPK; ATVASSTQKFQDLGVKNSEPSAR; RVELSGPKAAEPVSR; THMQNIKDITSSIHFEAYR; FINDQYEKYLQEEVNINR; RTEITIVKPQESAHR |
| P50454 | SERPH_HUMAN | 6 | 24.88 | 189.89 | DQAVENILVSPVVVASSLGLVSLGGK; AVLSAEQLRDEEVHAGLGELLR; HLAGLGLTEAIDKNKADLSR; LFYADHPFIFLVR; GVVEVTHDLQK; SSKQHYNCEHSK |
| P23246 | SFPQ_HUMAN | 2 | 11.66 | 104.18 | NLPQYVSNELLEEAFSVFGQVER; AVVIVDDRGR |
| Q07955 | SRSF1_HUMAN | 12 | 58.47 | 248.85 | SGGGVIRGPAGNNDCR; IYVGNLPPDIR; KLDNTKFR; SHEGETAYIR; TKDIEDVFYK; EAGDVCYADVYRDGTGVVEFVR; YGAIRDIDLK; KEDMTYAVR; FRSHEGETAYIR; GGPPFAFVEFEDPRDAEDAVYGR; TKDIEDVFYKYGAIR; DIEDVFYKYGAIRDIDLK |
| Q01130 | SRSF2_HUMAN | 7 | 32.58 | 191.23 | SYGRPPPDVEGMTSLKVDNLTYR; YGRPPDSHHSR; DRYTKESR; RVFEKYGR; VGDVYIPR; VGDVYIPRDR; TSPDTLRR |
| P84103 | SRSF3_HUMAN | 7 | 57.93 | 229.06 | VYVGNLGNNGNKTELER; VRVELSNGEKR; AFGYYGPLR; VRVELSNGEK; NRGPPPSWGR; NPPGFAFVEFEDPRDAADAVR; NPPGFAFVEFEDPRDAADAVRELDGR |
| Q13247 | SRSF6_HUMAN | 7 | 31.4 | 209.68 | QAGEVTYADAHKER; TNEGVIEFR; ALDKLDGTEINGR; LIVENLSSR; DRDGYSYGSR; RDRDGYSYGSR; TSGRDKYGPPVR |
| Q16629 | SRSF7_HUMAN | 4 | 26.47 | 197.62 | VYVGNLGTGAGKGELER; AFSYYGPLR; NPPGFAFVEFEDPRDAEDAVR; SRFDRPPAR |
| Q13242 | SRSF9_HUMAN | 2 | 11.39 | 115.96 | KLDDTKFR; SHEGETSYIR |
| P61764 | STXB1_HUMAN | 7 | 17.17 | 213.35 | SSASFSTTAVSAR; AAHVFFTDSCPDALFNELVK; HKHIAEVSQEVTR; ISEQTYQLSR; VKEVLLDEDDDLWIALR; LAEQIATLCATLKEYPAVR |
| Q15785 | TOM34_HUMHig hlights:
|
2 | 8.41 | 111.68 | SSFADISNLLQIEPR; NRVPSAGDVEK |
| Q12931 | TRAP1_HUMAN | 2 | 4.26 | 63.04 | GVVDSEDIPLNLSR; YVAQAHDKPR |
| P47985 | UCRI_HUMAN | 3 | 19.71 | 100.47 | SGPFAPVLSATSR; ESLSGQAVR; GVAGALRPLVQATVPATPEQPVLDLKRPFLSR |
| P67809 | YBOX1_HUMAN | 3 | 24.1 | 67.89 | GAEAANVTGPGGVPVQGSK; NEGSESAPEGQAQQR; AADPPAENSSAPEAEQGGAE |
The Table indicates 53 putative COXIV zipcode binding proteins identified by mass spectrometry analyses. The accession number, gene symbol, number of unique peptides identified, percentage of sequence coverage identified from MS/MS data, protein score and a list of sequences are indicated. The protein score according to the analyses software (PEAKS 7; Thermo Scientific) is defined as calculated weighted sum of the −10logP scores of the protein’s supporting peptides after removing any redundant peptides. * This asterisk denotes the explanation noted here as a footnote to the table.