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. Author manuscript; available in PMC: 2018 Apr 15.
Published in final edited form as: Methods. 2016 Oct 17;118-119:24–40. doi: 10.1016/j.ymeth.2016.10.007

Fig. 2.

Fig. 2

Nuclease treatment is a critical step in PAR-CLIP experiments. A) Phosphorimage of an SDS gel that resolved 32P-labeled RNA-FLAG/HA-SSB/La immunoprecipitates (IPs) prepared from untreated lysates and lysates treated with different concentrations (0, 0.1 and 1 U/µl) of either RNase A or RNase T1. M, molecular weight marker. B) Phosphorimage of recovered crosslinked RNAs (from A) after proteinase K digestion and 15% urea gel electrophoresis. Synthetic and 5’ radiolabeled RNAs of 19 and 35 nt in length served as size markers (M).