Skip to main content
. Author manuscript; available in PMC: 2018 Apr 15.
Published in final edited form as: Methods. 2016 Oct 17;118-119:24–40. doi: 10.1016/j.ymeth.2016.10.007

Fig. 3.

Fig. 3

Choice of magnetic beads impacts IP efficiency. Upper panel: Phosphorimage of the SDS gel that resolves 32P-labeled RNA-FLAG/HA-SSB/La IPs using ANTI-FLAG M2 antibody freshly coupled to Dynabeads (Dyna) or commercially pre-conjugated ANTI-FLAG M2 Magnetic Beads (Sigma). Lower panel: Corresponding immunoblot probed with an anti-HA antibody. M, molecular weight marker.