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. 2017 Apr 18;8:650. doi: 10.3389/fmicb.2017.00650

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Copyright © 2017 Giangrossi, Giuliodori, Tran, Amici, Marchini and Falconi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VirF binds the GH1 domain of RnaG through the preferential recognition of a signature sequence. EMSA was carried out, as a function of the indicated VirF concentrations, using the following ³²P-labeled RNAs: M129 and M130 (A); M129-130 duplex (B); GR4-48 and GR49-80 (C). Signals associated to bound RNAs were quantified and expressed as percentage of total radioactivity (D).