Table 4.

Confirmation of RNA sequencing results by real-time RT-PCR.

	Gene name	Rat colonized with multi-species culture		Rat colonized with MRSA single species		Rat colonized with PA single species
		Fold change in RNA seq	Fold change in real-time PCR	Fold change in RNA seq	Fold change in real-time PCR	Fold change in RNA seq	Fold change in real-time PCR
1	RPS9	4.27	3.23	3.72	1.87	3.97	2.71
2	MMP12	2.67	1.34	4.2	1.11	2.51	2.0
3	ITGB2	2.02	25.28	2.42	12.55	2.15	7.36
4	CST7	3.09	7.2	3.12	13.45	2.09	1.7
5	SCTR	4.26	15.78	5.18	20.11	4.13	18.25
6	APOC1	3.4	11.55	3.95	4.99	2.88	2.9
7	CD69	3.36	12.04	3.44	14.32	2.71	1.7
8	CCNO	2.8	5.6	3.68	11.31	3.74	3.78
9	KERA	−9.37	−5.55	−2.19	−7.69	−5.66	−6.6
10	GPD1	−6.4	−6.66	−1.62	−8.33	−6.1	−5.82
11	EEF1A2	−7.31	−3.3	−1.36	−4.76	−6.93	−1.5
12	HRC	−6.77	−4	−1.62	−6.2	−6.73	−5.8
13	FOXD1	−4.94	−4.34	−2.16	−8.33	−4.37	−6.25
14	MYH1	−10.9	−3.33	−2.36	−4.76	−10.5	−4
15	SYNPO21	−5.98	−5.26	−1.42	−7.1	−5.59	−3.84
16	TNMD	−7.12	−9	−1.88	−8.3	−7.3	−7.69
17	MYOM2	−7.1	−7.6	−2.61	−5.2	−6.65	−4
18	ACTA1	−11.5	−5.55	−1.36	−16.66	−10.1	−2.70