Figure 2. SGC-exosomes transport EGFR into liver cells.
(a) Electron microscope scanning of exosomes isolated from the medium of SGC7901 cells. (b) EGFR expression in both SGC exosomes and SGC7901 cells treated with EGFR siRNA. (c) Schematic description of the experimental design. The SGC exosomes were isolated and 50 μg exosomes were used to culture with 5 × 105 primary liver cells. (d) Confocal microscopy image of the internalization of fluorescently labelled exosomes in mixed liver cells. Scale bars, 50 μm. (e) SGC-exosome-mediated EGFR is located in the membrane of mixed primary liver cells. Scale bars, 50 μm. (f) Nanoparticle Tracking Analysis (NTA) of isolated exosomes. The data represent the mean±s.e.m. **P<0.01 (Student's t-test).