FIG 1.

Gs-DREADD-Luc mice express Gs-DREADD (GsD) in liver after Cre recombinase expression. (A) Schematic of the ROSA26-LSL-GsDREADD-CRE-luc allele. Arrowheads, PCR primers; arrows, transcriptional start sites. (B) Genomic PCR showing liver-specific recombination of the ROSA26-GsD locus after AAV-TBG-Cre infection in vivo compared with AAV-TBG-GFP infection (lane 1). PCR products correspond to intact and recombined LSL cassettes amplified by the primers indicated in panel A. Ctl, Creb1 genomic DNA (control). (C) Western blot analysis of GsD (anti-GFP) and HSP90 in livers of mice infected with AAV-TBG-GFP or AAV-TBG-Cre (n = 3 per group). (D) [³H]QNB binding assay on membranes isolated from livers of GsD mice infected with AAV-TBG-GFP or AAV-TBG-Cre (means ± standard errors of the means; n = 3 per group) (*, P < 0.05; **, P < 0.01; ***, P < 0.001 [by a t test]).