FIG 5.

Pcbp1 binds to the C-ISE region through the KHI domain. (A) Schematic diagram of Pcbp1 and its domains. Residue numbers and domains are indicated. (B) EMSA with wt or xm probe (sequences are given in Fig. 2A) and purified Pcbp1. The left panel shows Coomassie blue staining for Pcbp1/GST. The middle panel shows results of an EMSA with wt or xm probe and nuclear extracts (NE) or increasing amounts of Pcbp1. −, probe alone. The right panel represents competition analyses employing a 10- or 25-fold molar excess of unlabeled wt or xm RNA. (C) EMSA with wt probe and purified individual KH domains of Pcbp1. The left panel shows Coomassie blue staining for GST fusions of Pcbp1 KH domains. The right panel shows RNA-Pcbp1 KH domain protein complexes formed on wt probes. B, RNA-protein complex; P, probe.