FIG 6.

Combined TIA1 and Pcbp1 action on U-ISE and C-ISE is critical for exon 16 splicing. (A) Schematic diagram of the WT, Xm, and Up exon 16 minigenes showing replaced sequences at positions −15 to −24. (B and C) Splicing patterns of WT, Xm, or Up in the presence of vector (V), TIA1 (T), Pcbp1 (P), or both TIA1 and Pcbp1 (TP). MEL or HeLa cells were transfected with the indicated constructs and analyzed for exon 16 expression. E16 inclusion was calculated as the percentage of total RNA products containing exon 16. Averages and SDs were obtained for three independent experiments (n = 6), and results are presented at the bottom of each lane and as a bar graph. Anti-HA antibody detected HA-TIA1, and anti-Flag antibody detected Flag-Pcbp1. Actin served as a loading control.