FIG 4.

USP20 physically interacts with ERK3. (A) Lysates of exponentially proliferating 293T cells were subjected to immunoprecipitation (IP) with anti-USP20 or anti-HA antibody, which was used as a control. Immunoprecipitated proteins were analyzed by immunoblotting with the indicated antibodies. (B) 293T cells were transfected with full-length ERK3 or the truncated ERK3Δ(1–365) mutant comprising only the kinase domain. Cell lysates were immunoprecipitated with anti-USP20 or anti-HA antibody and analyzed by immunoblotting.