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. 2017 Apr 12;8:14809. doi: 10.1038/ncomms14809

Figure 2. Cognate interaction with islet cells contributes to CD8+ T-cell expansion at the site of inflammation.

Figure 2

Flow cytometry analysis of OT-I cells (viable CD45.1+CD8+Vα2+ gate) 6 days post graft. (a) Total divided OT-I in the draining renal LN and graft after receipt of a single graft of 400 B6.βOVA or bm1.βOVA islets. Data for B6.βOVA grafted mice are a subset of that shown in Fig. 1b. Each point represents an individual mouse. Pooled data from two independent experiments are shown in each panel: n=5 recipients of B6.βOVA grafts, and n=6 recipients of bm1.βOVA grafts. Horizontal bars are means, P values were calculated by unpaired, two-tailed t-test with Welch's correction. (b) Schematic of bipolar graft model in which grafts of 200 islets are placed at opposite poles of the same kidney and share a common draining renal LN. Total divided OT-I in (c) B6.βOVA and KbKO.βOVA bipolar grafts and (d) B6.βOVA and B6 bipolar grafts (n=5 recipient mice pooled from two independent experiments in each of c and d) Data for the same mouse are connected by dashed lines: P values were calculated by ratio paired t-test.