Figure 3. Cognate interaction with IRL does not significantly contribute to CD8⁺ T-cell expansion.

Flow cytometry analysis showing depletion of CD11c⁺ IRL in CD11c.DTR.GFP mice treated with DT: (a) Representative plots showing gating of CD11c⁺IRL in islets from untreated control mice and their depletion on the day following a single DT treatment, and (b) enumeration of CD11c⁺IRL in untreated and DT treated mice. Each point represents an individual islet preparation containing pooled islets from 1 to 8 mice, n=5 untreated islet preparations and 6 DT treated islet preparations pooled from four independent experiments. P value calculated by unpaired, two-tailed t-test with Welch's correction. (c) Total divided OT-I at 6 days post bipolar graft of islets obtained from CD11c.DTR.GFP.βOVA mice that were untreated or treated with DT (n=6 recipient mice pooled from two independent experiments). Data for the same mouse are connected by dashed lines; P values calculated by ratio paired t-test. (d) Representative plots showing absence or presence of H-2K^b expression on CD11c⁺IRL of KbKO mice reconstituted with KbKO or B6 BM respectively. Pregated on viable cells. (e) Total divided OT-I at 6 days post bipolar graft of islets obtained from chimaeric mice (n=3 recipient mice from a single experiment). Data for the same mouse are connected by dashed lines; P values calculated by ratio paired t-test.