Figure 4. Cognate interaction with islet parenchymal β cells contributes to CD8⁺ T-cell expansion.

(a) Flow cytometry plots (gated on viable cells) showing selective restoration of H-2K^b expression on parenchymal cells. Histograms represent islets from KbKO (shaded), B6 (dashed) or KbKO.βKb (bold) mice. (b) Total divided OT-I at 6 days post bipolar graft of KbKO.βOVA and KbKO. βOVA.βKb. islets (n=6 recipient mice pooled from three independent experiments). Data for the same mouse are connected by dashed lines; P values calculated by ratio paired t-test.