FIG 1.

Reduction of iron citrate (10 mM initial concentration) in cultures of Desulfitobacterium hafniense strains TCE1 and LBE in the presence of chlorine. Three concentrations of NaCl (0, 0.2, and 0.5%) were compared. Iron reduction was evaluated by the production of Fe(II) at 0, 1, and 2 days (T0, T1, and T2, respectively) of incubation after the addition of the Fe(III) source. Abiotic controls were performed for each NaCl concentration tested. Fe(II) values are the averages and standard deviations from three independent replicates. A change in the appearance of the TCE1 and LBE cultures compared to the abiotic control is shown on the right-hand side of the figure (example for the cultures at 0% NaCl after 2 days of incubation).