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(A and C) Proportion of total type IV pilin reads that clustered with long type IV pili or e-pili in metagenomic (A) and metatranscriptomic (C) libraries. (B and D) Taxonomic assignment of e-pilin reads in metagenomic (B) and metatranscriptomic (D) libraries. Results represent an average of values obtained from nhmmer, BLASTx, and Bowtie2 alignments of QC-filtered merged reads.
