TABLE 1.
Hemotropic mycoplasmas detected in raccoons
| GenBank accession no.a | Sample ID | Hemoplasma genotype detected in raccoons | Primer set used for full-length 16S rRNA amplification (see Table S1) | Sequence homology to other hemoplasmas (GenBank accession no.) |
|---|---|---|---|---|
| KC920443 | PRLO50 | 3 | 16S-PCR-1 | 96% to “Candidatus Mycoplasma erythrodidelphis” (AF178676) |
| KC920441 | PRLO49 | |||
| KC920447 | PRLO72 | |||
| KC920446 | PRLO84 | |||
| KC920445 | PRLO62 | |||
| KC920444 | PRLO57 | |||
| KC920442 | PRLO42 | |||
| KC920448 | PRLO53 | |||
| KC920440 | PRLO25 | |||
| KC920439 | PRLO96 | |||
| KF743729 | PRLO102 | 2 | 16S-PCR-3 | 92–93% to “Candidatus Mycoplasma haemozalophi” (GU905012) and “Candidatus Mycoplasma haemolamae” (AF306346) |
| KF743724 | PRLO88 | |||
| KF743727 | PRLO92 | |||
| KF743722 | PRLO86 | |||
| KF743713 | PRLO56 | |||
| KC936280 | PRLO87 | |||
| KF743717 | PRLO65 | |||
| KF743726 | PRLO91 | 4 | 16S-PCR-2 | 86–88% to raccoon hemoplasma genotypes 2 and 3, and to “Candidatus Mycoplasma haemominutum,” M. wenyonii, and M. ovis |
| KF743711 | PRLO46 | |||
| KF743728 | PRLO101 | |||
| KF743721 | PRLO84 | |||
| KF743719 | PRLO77 | |||
| KF743718 | PRLO74 | |||
| KF743716 | PRLO64 | |||
| KF743706 | PRLO24 | 5 | 16S-PCR-5 | 96–97% to M. haemocanis (AY529641) (including M. haemocanis detected in Japanese raccoon dog [AB848714] and M. haemocanis/M. haemofelis-like sp. detected in Japanese black bear [AB725596]), to M. haemofelis (AF548631), and to raccoon hemoplasma genotype 1 |
| KF743734 | PRLO_56HC | |||
| KF743715 | PRLO62 | |||
| KF743736 | PRLO_103HC | |||
| KF743704 | PRLO14 | |||
| KF743710 | PRLO44 | 6 | 16S-PCR-8 | 91–92% to raccoon hemoplasma genotype 5, and to “Candidatus Mycoplasma haemobos” (EF460765) |
| KF743733 | PRLO_55HB | |||
| KF743707 | PRLO33 | |||
| KF743731 | PRLO104 | |||
| KF743720 | PRLO80 | |||
| KF743714 | PRLO59 | 1 (M. haemocanis/M. haemofelis-like sp.) | 16S-PCR-5 | 99% to M. haemocanis (AY529641) and M. haemofelis (AF548631) |
| KF743709 | PRLO40 | |||
| KF743723 | PRLO87 | |||
| KF743735 | PRLO_92HC | |||
| KF743732 | PRLO_43SC | |||
| KF743705 | PRLO21 | |||
| KF743712 | PRLO55 | |||
| KF743725 | PRLO90 | |||
| KF743708 | PRLO38 | |||
| KF743730 | PRLO103 |
a
The reference sequence for each hemoplasma genotype is indicated in bold. The numbering of raccoon hemoplasma genotypes (1 to 6) is based on their sequential discovery.