TABLE 2.

Treatment effects on community structure for S1 and S2

Treatment	S1 P valuea		S2 P valuea
	Parsimony	AMOVA	Parsimony	AMOVA
Gelling agent	0.003	<0.001	0.002	<0.001
Medium	0.6	0.07	<0.001	<0.001
Incubation time	0.5b	0.6b	0.6–0.9c	0.07–0.09c
Incubation temp	0.5b	0.6b	0.03	0.07
AHLs (0.5 μM)	NAd	NA	0.08	0.03
AHLs (50 μM)	NA	NA	0.07	0.003

^aParsimony tests were performed on weighted UniFrac distances, and analyses of molecular variance (AMOVA) were performed on Bray-Curtis distances.

^bTime and temperature comparisons were identical, as all plates incubated at 10°C were incubated for 10 weeks and all plates incubated at 25°C were incubated for 5 weeks.

^cTwo different incubation times were applied for each temperature (10°C for 5 weeks and 10 weeks; 25°C for 2 weeks and 5 weeks).

^dNA, not applicable.