TABLE 3.
Strains and plasmids used in this study
| Strain or plasmid | Characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| S. wittichii DP58 | Degrades phenazine-1-carboxylic acid; Smr | Laboratory stock |
| S. wittichii RW1 | Degrades dibenzo-p-dioxin and phenazine-1-carboxylic acid; Smr | 11 |
| P. putida KT2440 | Wild type | ATCC 12633 |
| S. yanoikuyae XLDN2-5 | Degrades carbazole | 55 |
| E. coli DH5α | Host strain for cloning vectors, F− recA1 endA1 thi-1 supE44 relA1 deoR Δ(lacZYA-argF)U169 Ф80dlacZΔM15 | Invitrogen |
| E. coli BL21(DE3) | Host strain for pET28a | Invitrogen |
| Plasmids | ||
| pMD18T | Cloning vector; Ampr | TaKaRa |
| pET28a(+) | Expression vector; Kmr | Novagen |
| pBBRMCS-5 | Broad-host-range cloning vector; Gmr | 56 |
| pME6015 | pVS1-p15A E. coli_Pseudomonas shuttle vector for translational lacZ fusions and promoter probing; Tcr | 49 |
| pHN1257 | Vector with Ptrc promoter; Kmr | 57 |
| pMDpcaA1A2A3A4 | pMD18T derivative carrying pcaA1A2A3A4; Ampr | This study |
| pMDpcaA1A2 | pMD18T derivative carrying pcaA1A2; Ampr | This study |
| PMDpcaA1A2A4 | pMD18T derivative carrying pcaA1A2A4; Ampr | This study |
| pBBRpcaA1A2 | pBBR1MCS-5 derivative carrying pcaA1A2; Gmr | This study |
| pBBR2S | Broad-host-range cloning vector with Ptrc promoter from pHN1257; Kmr | This study |
| pBBR2SHpcaA1A2 | pBBR2S derivative carrying pcaA1A2 and His6 coding sequence; Kmr | This study |
| pETpcaA1A2 | pET28a(+) derivative carrying pcaA1A2; Kmr | This study |
| pETpcaA3 | pET28a(+) derivative carrying pcaA3; Kmr | This study |
| pETpcaA4 | pET28a(+) derivative carrying pcaA4; Kmr | This study |
| pMDpcaB1B2 | pMD18T derivative carrying pcaB1B2; Ampr | This study |
| pMEpcap | pME6015 with the promoter sequence of pcaA1A2; Tcr | This study |
| pETRedA2 | pET-29a(+) derivative carrying redA2; Kmr | 14 |
| pETpcaC | pET-29a(+) derivative carrying pcaC; Kmr | This study |
a
Smr, streptomycin resistance; Ampr, ampicillin resistance; Kmr, kanamycin resistance; Gmr, gentamicin resistance; Tcr, tetracycline resistance.