FIG 2.
MazF1Bif is an mRNA interferase that is inhibited by its cognate antitoxin, MazE1Bif. Relative transcript levels of tufABif were determined in E. coli expressing tufABif with pACYCDuet-1, pAD-F1, or pAD-F1E1. The strains were grown with 0.2% arabinose for 2 h to induce tufABif expression. Then, 1 mM IPTG was added to induce MazF1Bif or MazF1Bif and MazE1Bif expression. After 3 h, 200 μg/ml rifampin was added. Samples were collected at the indicated time points after rifampin addition. The levels of tufABif mRNA were monitored by qRT-PCR (normalized to the 16S rRNA transcript level). The values presented are the averages from three independent experiments, and error bars represent the standard deviations. A two-way analysis of variance with Bonferroni posttest was used to obtain P values for each time point: a, P < 0.05 versus pACYCDuet-1; b, P < 0.05 versus pAD-F1E1.