. 2017 Apr 17;83(9):e03232-16. doi: 10.1128/AEM.03232-16

TABLE 2.

Oligonucleotide primers used in PCR and qRT-PCR

Gene	Assay	Direction	Primer sequence^a	Length (bp)	Source or reference
mazF₁^Bif	PCR	Sense	CCGGAATTCAATGAAACGAGGTGAGATT	324	This work
mazF₁^Bif	PCR	Antisense	CCCAAGCTTTCACAGCAAACCTAGAAC	324	This work
mazF₁^Bif (primer mazF₁^Bif-2)	PCR	Sense	TTAAGAAGGAGATATACCATGGAAATGAAACGAGGTGAGATTCG	354	This work
mazF₁^Bif (primer mazF₁^Bif-2)	PCR	Antisense	CTCGAGTGCGGCCGCAAGCTTTTACAGATCCTCTTCTGAGATGAGTTTTTGTTCCAGCAAACCTAGAACCCG	354	This work
mazE₁^Bif	PCR	Sense	GGAAGATCTAATGAGCATACAGATTGCCA	246	This work
mazE₁^Bif	PCR	Antisense	CCGCTCGAGCCTCGTTTCATCGGACAT	246	This work
mazE₁^Bif (primer mazE₁^Bif-2)	PCR	Sense	GGATCCATGAGCATACAGATTGCCA	246	This work
mazE₁^Bif (primer mazE₁^Bif-2)	PCR	Antisense	CTCGAGCCTCGTTTCATCGGACAT	246	This work
mazE₂^Bif	PCR	Sense	GGAAGATCTTATGGCTATCAAGGAGAAGG	294	This work
mazE₂^Bif	PCR	Antisense	CCGCTCGAGGTCTTCATCATCGTCCCA	294	This work
relB^Bif	PCR	Sense	GGAAGATCTATTGTCTTACGTAATGATGTTGG	300	This work
relB^Bif	PCR	Antisense	CCGCTCGAGGATTCCCAACGAATCGAA	300	This work
mazE^Bif	qRT-PCR	Sense	GCTGCGTTGTTTAAGGAGAC	94	10
mazE^Bif	qRT-PCR	Antisense	ACCCACGGTAATCATTGAAC	94	10
tufA^Bif	qRT-PCR	Sense	ATCCGTCCGACCCAGACC	123	54
tufA^Bif	qRT-PCR	Antisense	CTCGACATCCTCACGGCC	123	54
16S	qRT-PCR	Sense	TACGGGAGGCAGCAG	191	55
16S	qRT-PCR	Antisense	ATTACCGCGGCTGCTGG	191	55

Restriction sites for XhoI, EcoRI, BglII, and HindIII incorporated into the primers are in boldface, and the sequence for the Myc tag is underlined.