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EQAPOL sample amplification using optimized protocol.
| Subtypes | Sensitivity by VL (copies/mL)∗ | ||||||
|---|---|---|---|---|---|---|---|
| 5000 | 2500 | 1000 | 500 | 200 | 100 | 50 | |
| A1 | +++ | +++ | +++ | +++ | +++ | +++ | +−− |
| B | +++ | +++ | +++ | +++ | +++ | +++ | −−+ |
| C | +++ | +++ | +++ | +++ | +−− | −+− | −−− |
| D | +++ | +++ | +++ | −−− | +−+ | −−− | −+− |
∗Each +/− sign represents an individual run of the same sample type, run in triplicate.
