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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2017 Feb 28;105:38–48. doi: 10.1016/j.yjmcc.2017.02.005

Figure 5.

Figure 5

IL-19 reduces LDLRAP1 expression. A. LDLRAP1 mRNA expression is significantly reduced by IL-19. Human VSMC were treated with IL-19, RNA extracted at the indicated times, and LDLRAP1 mRNA abundance detected by quantitative RT-PCR. B. IL-19 significantly reduces LDLRAP1 protein expression. VSMC lysates were harvested at the indicated times post IL-19 stimulation and western blotted using the indicated antibodies. C. Densiometric quantitation of LDLRAP1 protein in VSMC treated with IL-19. Times from 4 to 48 hours were significantly lower compared with untreated cells. D. anti-miR133a prevents IL-19 from reducing LDLRAP1 protein abundance. 25ug anti-miR133a or scrambled control were transfected into human VSMC and stimulated with IL-19. E. densiometric quantitation of LDLRAP1 protein expression in VSMC transfected with anti-miR133a. Asterisk indicates significant reduction in the scrambled versus anti-miR transfected VSMC. * indicate P<0.05, **P<0.01, *** P<0.001.