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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Mol Cell Cardiol. 2017 Feb 28;105:38–48. doi: 10.1016/j.yjmcc.2017.02.005

Figure 8.

Figure 8

miR133a and LDLRAP1 regulate human VSMC proliferation. A. miR133a reduces VSMC proliferation. Human VSMC were transduced with 4 MOI of Adscrambled control vector or AdmiR133a, equal numbers seeded, and VSMC counted at 3 and 6 days post-seeding. B. Relative expression of miR133a in adenoviral transduced VSMC. VSMC were infected with AdmiR133a or Adcontrol vector, RNA isolated and miR133a abundance detected by quantitative RT-PCR. C. LDLRAP1 knockdown reduces VSMC proliferation. VSMC were transfected with LDLRAP1 or scrambled siRNA, equal numbers seeded, and VSMC counted at 3 and 6 days postseeding with a BioRad TC20 automated cell counter, n=3. D. western blot verifying that LDLRAP1 siRNA reduces LDLRAP1 protein expression in human VSMC. Asterisks indicate significant reduction in the treated versus control VSMC. E. miR133a levels are significantly increased in plasma from hyperlipidemic patients. Plasma was isolated from normal, or patients with plasma cholesterol >200mg/dl. RNA was isolated, and miR133a levels quantitated by quantitative RT-PCR. N=9 each. ** indicate P<0.01, *** P<0.001.