Figure 2.

Securinine inhibits LPS-induced NO production in the BV2 cell line and in mouse primary microglia challenged with LPS exposure. BV2 microglial cells (a) and mouse primary microglia (b) were pretreated with securinine (2, 5, and 10 μM) for 1 h and then stimulated with LPS (500 ng/mL) for 24 h. NO levels in the medium were determined using the Griess Reagent system. The data are expressed as mean ± SD, n = 4. ^∗P < 0.01, significantly different from control samples. ^∗∗P < 0.05 significantly different from the LPS-treated group alone.