Figure 8.

Securinine pretreatment inhibits LPS-induced NO production in RAW 264.7 macrophage cells and in primary astrocyte cultures. RAW 264.7 macrophage cells or primary astrocyte cultures (10 × 10⁴ cells/well in 48-well plates) were incubated with 500 ng/mL of LPS or LPS/IFN-γ (50 unit/mL) in the presence or absence of securinine (10 μM) for 24 h. The amount of nitrite secreted into the supernatant was measured using the Griess Reagent assay ((a) RAW 264.7 cells, ^∗∗∗P < 0.05 versus control; (b and c) primary astrocyte cultures). The data were expressed as the mean ± SD (n = 3) and are representative of three independent experiments. ^∗P < 0.05 and ^∗∗P < 0.01, significantly different from cells treated with LPS only.