Table 2. Functional parameters of WT CaSR and the trans-activation response arising from the S170A:F801A heterodimer.

	EC50 (mM)	pEC50 ± S.E.M.	Max ± S.E.M. (%)a	Hill coefficient ± S.E.M.
HA-CaSR WT + myc CaSR-WT	2.98	2.53 ± 0.02	100	4.24 ± 0.19
HA-S170A + myc-F801A	12.7	1.90 ± 0.03	30 ± 1.9	2.69 ± 0.19

Ca²⁺-mediated activation of the G_q signaling pathway was measured using the IP-One assay, and data are means ± S.E.M. of three independent experiments performed in triplicates.

^aThe maximum trans-activation response is normalized to the maximum response of WT CaSR.

EC₅₀: the concentration of Ca²⁺ that is required for eliciting 50% of the maximum response. pEC₅₀ = −log(EC₅₀).