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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Cell Biol Toxicol. 2016 May 17;32(4):347–358. doi: 10.1007/s10565-016-9339-8

Fig. 5.

Fig. 5

Mesd binds to POS vesicles and apoptotic cells. a Mesd-FLAG or GFP-FLAG cell lysate without detergent was incubated with POS vesicles. After washing, bound FLAG-tagged proteins were detected by flow cytometry using Alexa Fluor 594-labeled anti-FLAG antibody. b,c Mesd binds to apoptotic but not healthy cells. Apoptotic and healthy HEK293 cells were incubated with FITC-labeled MBP-Mesd or MBP, washed and analyzed by flow cytometry (b) or confocal microscopy (c). Apoptotic cells were labeled with propidium iodide. Confocal z-stack images showed that FITC-Mesd bound to the surface of apoptotic cells like rings in c (Zoom-in, middle row). Bar = 25 μm.