Table 1. Fluorescent dyes tested.
Dye | Supplier | Abs. (nm) | Em. (nm) | τ (ns) | Channel |
---|---|---|---|---|---|
580CP | ref. 27 | 583 | 606 | 3.6 (ref. 27) | APD1 |
Atto594 | ATTO-TEC | 601 | 627 | 3.2 (ref. 28) | APD1 |
Abberior Star600 | Abberior | 604 | 627 | 1.2 | APD1 |
620CP | ref. 27 | 618 | 646 | 1.0 (ref. 27) | APD2 |
DyLight633 | Thermo Fischer Scientific | 623 | 649 | 1.7 | APD2 |
Abberior Star635P | Abberior | 633 | 654 | 3.0 (ref. 28) | APD2 |
SiR | ref. 26 | 650 | 674 | 2.7 (ref. 27) | APD3 |
KK1441 | ref. 24 (compound 1a-H) | 661 | 679 | 2.4 (ref. 24) | APD3 |
CF680R | Biotium | 680 | 701 | 1.8 (ref. 28) | APD4 |
Absorption (Abs.) and emission maxima (Em.) in nm as specified by the supplier, together with experimentally measured lifetimes (τ) in ns and main detection channel, in which the majority of signal is collected.