Figure 2. Antibody responses following a prime-boost immunization with viral-vectored (vv) vaccines (ChAd63 followed by MVA) expressing different versions of PvCSP.

Outbred CD-1 mice (n = 6) were immunized with vv expressing various versions of PvCSP (N210/N247C, N210C, N247C, NC; see Fig. 1) using a prime-boost interval of 8 weeks. Control groups were mock-vaccinated with vv expressing P. berghei CSP and naive mice were injected with PBS, and did not induce any antibodies. Serum was collected every two weeks and antibody titers –total IgG- were quantified using a standardized ELISA against the PvCSP VK210 and VK247 antigens.