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. 2017 Apr 18;7:46482. doi: 10.1038/srep46482

Figure 5. Immunofluorescence assay (IFA) to assess expression of PvCSP on the surface of the transgenic sporozoites.

Figure 5

Sporozoites from mosquito salivary-glands were stained with anti-PvCSP-210 (MR4) diluted 200 times, anti-PvCSP-247 (MR4) diluted 200 times, and anti-PbCSP (3D11) diluted 1000 times (3D11 stock (1000X) concentration was at 10 mg/ml and final working concentration was 10 μg/ml). Alexa Fluor® 488-labelled anti-mouse IgG and Hoechst-33342 were also included for the assay. (A) Transgenic parasite line 2196cl1 expressing the PvCSP VK210; (B) Transgenic parasite line 2199cl1 expressing the PvCSP VK247; (C) Wild type P. berghei parasite line 676m1cl1 showing expression of PbCSP.