Table 1. Bacterial strains.
Strain | Description | Antibiotic Resistancea | Source (Reference) |
---|---|---|---|
J166 | Wild Type | 35 | |
J99 | Wild Type | 78 | |
26695 | Wild Type | 36,78 | |
J166ΔbabAupstream | J166 with the upstream portion of babA replaced by CAT_rpsL (transformed with pBabA) | Cm | This study |
J166sc | Single colony isolate of J166 | Str | This study |
J1667CT | J166 engineered with 7 CT repeats in the 5′ end of babA | Str | This study |
J1668CT | J166 engineered with 8 CT repeats in the 5′ end of babA | Str | This study |
J166ΔbabA | J166 with babA replaced by CAT_rpsL | Cm | 20 |
J166 BabACL2 | J166 with Cys 189 and 197 in BabA replaced with Ala | Str | 20 |
J166 BabACL28CT | J166BabACL2 engineered to have 8 CT repeats in the 5′ end of babA, non-binding BabA is expressed | Str | This study |
J166 BabACL29CT | J166BabACL2 engineered to have 9 CT repeats in the 5′ end of babA, BabA is not expressed | Str | This study |
aCm, chloramphenicol; Str, streptomycin.