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. 2017 Apr 18;7:46457. doi: 10.1038/srep46457

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Copyright © 2017, The Author(s)

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All pictures were obtained under 20X magnification (n = 5 per group). (A) Safranin O/Fast Green staining shows the extent of proteoglycan loss among the different diet groups both in non-IL-1β treated and IL-1β treated pellets. (B) Quantification of sGAG release in the supernatant was performed by DMB assay, which showed that PA and SA-treated pellets exhibited a trend of decreased chondrogenesis irrespective of IL-1β treatment. (C,D) Graph showing the increase in sGAG release in response to increasing concentration of PA and SA. All values are represented as mean ± SD. Scale bar is 100 μm (P < 0.05).