Table 3. Oligonucleotide sequences for primers used in dsRNA construct design.

Target genes	Forward primer	Reverse primer
SchgrETHR	5′-TAATACGACTCACTATAGGGAGA CGTCATCATCATGAGCATC-3′	5′-TAATACGACTCACTATAGGGAGA GGCGAGCAGACAGATGAGC-3′
SchgrETHpre	5′-TAATACGACTCACTATAGGGAGA TGGTCCTGGTGGCGGCA-3′	5′-TAATACGACTCACTATAGGGAGA TTTGTGCGGCGGCCAATC-3′
SchgrEcR	5′-GAAATTAATACGACTCACTATAGGGCC ACGTGAGGTTTCGGCACATC-3′	5′-GAAATTAATACGACTCACTATAGGGCC GTTTCCCCCATACCAGCCAG-3′
SchgrRXR	5′-GAAATTAATACGACTCACTATAGGGCC GCTCAATGGGTCCACAGTCA-3′	5′-GAAATTAATACGACTCACTATAGGGCC ACACCATAATGCTTCCCGCT-3′
GFP	5′-TAATACGACTCACTATAGGGAGA AAGGTGATGCTACATACGGAA-3′	5′-TAATACGACTCACTATAGGGAGA ATCCCAGCAGCAGTTACAAAC-3′

Underlined sequences are the T7 promoter sequences.

Abbreviations: ETHR = ecdysis triggering hormone receptor, ETHpre = ecdysis triggering hormone precursor, EcR = ecdysone receptor, RXR = retinoid-X-receptor, GFP = green fluorescent protein.