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. 2017 Apr 5;13:1744806917701743. doi: 10.1177/1744806917701743

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Time course (a) and quantification analysis (b) showing that SNS-PKG-I^−/− mice displayed a significant reduction of formalin-induced spontaneous pain responses in both phases as compared to PKG-I^fl/fl mice. (c) Typical examples showing that phosphorylated ERK1/2 (pERK1/2) immunoreactivity was induced in the DRG (upper panels) and spinal cord (lower panels) at 1 h following intraplantar injection of formalin in PKG-I^fl/fl mice and SNS-PKG-I^−/− mice. (d) Quantitative summary of the number of pERK1/2-positive cells in the DRG and spinal cord derived from control and formalin-treated mice. (e) Typical examples showing that c-Fos immunoreactivity was induced in both the superficial and deep lamina of the spinal cord at 1 h following intraplantar injection of formalin in PKG-I^fl/fl mice and SNS-PKG-I^−/− mice (upper panels). Magnification of superficial (middle panels) and deep lamina (lower panels) are shown. (f) Quantitative summary of the number of c-Fos-positive cells in both the superficial and deep lamina of spinal cord derived from control and formalin-treated mice. * indicates statistically significant differences (P < 0.05) between PKG-I^fl/fl mice and SNS-PKG-I^−/− mice (ANOVA, post hoc Fisher’s test, n = 4 mice per genotype). Scale bars represent 100 µm in (c) and upper panels in (e), 50 µm in middle and lower panels in (e).