Fig. 2.

Schematic representation of the design of pCro replicon vector based on genome of croton yellow vein mosaic virus (CYVMV). a Amplification and cloning of i MCS cassette, ii intergenic region (IR) and iii partial genome of CYVMV (par-CYVMV) in pGEMT-easy vector. Template for PCR, primer name, their positions and expected amplicon sizes were depicted in the upper panel. b Sequential sub-cloning of i MCS cassette, ii IR and iii par-CYVMV from pGEMT vector to a blunted pCAMBIA2300 vector to develop pCro vector