Fig. 4.

PCR confirmation of the presence of genome of a CYVMV and b CroYVMB in agroinoculated Nicotiana benthamiana (NB), N. glutinosa (NG) and N. tabacum (NT) plants. M marker; lane 1 mock inoculated plant; lane 2 positive control for CYVMV and CroYVMB; lane 3–5 amplification of CYVMV (for a) or CroYVMB (for b) from NB, NG and NT plants, respectively inoculated with only CYVMV; lane 6–8 amplification of CYVMV (for a) or CroYVMB (for b) from NB, NG and NT plants, respectively inoculated with CYVMV and CroYVMB