Table 2.
Agroinfectivity analysis of the cloned DNA of croton yellow vein mosaic virus (CYVMV) and croton yellow vein mosaic betasatellite (CroYVMB) on different Nicotiana spp.
| Constructa | Plant | No. of symptomatic plants out of ten no. inoculated | dpi | Systemic symptoms | ||
|---|---|---|---|---|---|---|
| E1 | E2 | E3 | ||||
| CYVMV | NB | 10 | 8 | 8 | 10–13 | Leaf curling, leaf rolling and vein thickening |
| NG | 6 | 7 | 5 | 15–20 | Mild puckering on leaves | |
| NT | 2 | 1 | 4 | 30–35 | Vein clearing | |
| CYVMV + CroYVMB | NB | 10 | 10 | 9 | 8–10 | Severe leaf curling, leaf rolling, vein thickening and stunting |
| NG | 4 | 8 | 7 | 10–12 | Severe leaf curling with puckering, smalling of leaves, stunting | |
| NT | 4 | 7 | 6 | 25–30 | Puckering on leaves and stunting | |
| pC2300 (mock inoculation) | NB | 0 | 0 | 0 | – | No symptoms |
| NG | 0 | 0 | 0 | – | No symptoms | |
| NT | 0 | 0 | 0 | – | No symptoms | |
NB, Nicotiana benthamiana; NG, N. glutinosa; NT, N. tabacum; dpi, days post inoculation; E, experiment number
aCYVMV: a partial dimer was used, CroYVMB: croton yellow vein mosaic betasatellite dimer was used. Constructs were mobilized into Agrobacterium tumefaciens EHA105 strain