Fig. 2.

Endocrine-specific defects in the Pdx1^ΔII/− pancreas. (A,B) Overall morphology of the Pdx1^+/− and Pdx1^ΔII/− pancreatic/duodenal region at P1. (C-D′) Gcg and Pdx1 expression (single Pdx1 channel and merge) in P1 endocrine clusters. (E,F) Ins and Gcg expression at P1. Arrows indicate Ins⁺ Gcg⁺ co-expressing cells. Note that the large cluster of hormonal cells shown in F was not commonly found in Pdx1^ΔII/− and is shown for comparison. (G,H) Pdx1^2E3-lacZ reporter for Pdx1 transcriptional activities in E13.5 Pdx1^+/− and Pdx1^ΔII/− pancreata. (I-J′) Sox9 and Pdx1 expression at E13.5. (K-L′) Decreased Neurog3⁺ cell numbers are observed in E14.5 Pdx1^ΔII/− pancreas (L,L′). (M,N) Morphometric analysis of Sox9⁺ cells and Neurog3⁺ cells at E14.5. *P<0.05. (O) Ins⁺:Gcg⁺ cell ratios in Pdx1^+/− and Pdx1^ΔII/− at P1.