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The basic region determines Ascl2 function as an MRF inhibitor. (A,D) Luciferase assays with 4R-tk-luc as the reporter. Error bars represent the mean with s.d. of three independent biological replicates. (B) Amino acid alignment analysis of the bHLH domain of Ascl2 and three MRFs. Subdomains are indicated. (C) Schematic and sequencing results of the Ascl2 and MyoD chimeric bHLH domains.
