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. 2017 Jan 15;144(2):305–312. doi: 10.1242/dev.141135

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — CenpH is not required for spindle assembly. CenpH antibody was microinjected into GVBD stage oocytes. (A) Confocal images of control and CenpH antibody-injected oocytes immunostained for DNA, kinetochores (ACA), microtubule organizing center (γ-tubulin) and microtubules (α-tubulin). (B) Length:width ratios of spindles, maximal spindle lengths and spindle areas for control and CenpH antibody-injected oocytes at 2 h (n=20 and n=12), 4 h (n=20 and n=14) and 8 h (n=18 and n=14). (C) Confocal images of control and CenpH antibody-injected oocytes stained for DNA and immunostained for kinetochores (ACA) and microtubules (α-tubulin) at 8 h (n=18 and n=14). The interkinetochore distance of control and CenpH antibody-injected oocytes was assessed. Data are mean± s.e.m. The total numbers of analyzed oocytes are indicated (n). Scale bars: 20 μm.