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. 2017 Apr 19;8:443. doi: 10.3389/fimmu.2017.00443

Figure 5.

Figure 5

(A–D) Effect of Me-Indoxam or with Heparinase on human group V (hGV)-induced vascular endothelial growth factor (VEGF)-A, CXCL8/IL-8, Ang1, and VEGF-A165b release from PMNs. hGV (3 µg/ml) was preincubated (37°C, 20 min) with Me-Indoxam (0.1 µM) or control medium. PMNs were then incubated (37°C, 1 h) with heparinase (0.4 U/ml) or control medium and then stimulated (37°C, 30 min) with hGV alone or with the combination of hGV with Me-Indoxam. (E,F) αVβ3 (P11) and α4β1 (TCS2314) receptor antagonists inhibit GV-induced PMNs production of antiangiogenic factors. PMNs were preincubated (37°C, 30 min) with or without P11 and TCS 2314 (100 nM) and then stimulated (37°C, 30 min) with hGV (3 µg/ml). Data are the mean ± SD of eight different preparations of PMNs. *p < 0.05 vs. respective control. §p < 0.05 vs. hGV alone.