Table 1.
Primer sequences and conditions for quantitative PCR.
| Target | Product length (bp) | Ta (°C) | Primer (5′–3′) | GenBank accession no. or reference |
|---|---|---|---|---|
| VEGFA165a | 79 | 60 | Forward: GCCTTGCCTTGCTGCTCTAC | NM_003376 |
| Reverse: TGATTCTGCCCTCCTCCTTCTG | ||||
| VEGFA165b | 157 | 60 | Forward: GAGCAAGACAAGAAAATCCC | (43) |
| Reverse: GTGAGAGATCTGCAAGTACG | ||||
| VEGFB | 128 | 60 | Forward: AGGACAGAGTTGGAAGAGGAG | NM-003377 |
| Reverse: AGGAAGAGCCAGTTGTAAGATG | ||||
| VEGFC | 197 | 60 | Forward: ATGTTTTCCTCGGATGCTGGA | NM_005429 |
| Reverse: CATTGGCTGGGGAAGAGTTT | ||||
| VEGFD | 226 | 60 | Forward: GTATGGACTCTCGCTCAGCAT | NM_004469 |
| Reverse: AGGCTCTCTTCATTGCAACAG | ||||
| Ang1 | 73 | 60 | Forward: CAGGAGGATGGTGGTTTGATG | NM_001314051 |
| Reverse: TGGTTTTGTCCCGCAGTATAGAA | ||||
| Ang2 | 65 | 60 | Forward: TTCCTCCTGCCAGAGATGGA | NM_001118888 |
| Reverse: TGCACAGCATTGGACACGTA | ||||
| β-Actin | 99 | 60 | Forward: TGCGTGACATTAAGGAGAAG | NM_001101 |
| Reverse: GCTCGTAGCTCTTCTCCA |
Ta, annealing temperature.